Next Generation Sequencing

Next Generation Sequencing, based in Dr. Jordan’s Laboratory at The George Washington University, School of Public Health in the Science and Engineering Hall, provides NextGen Sequencing assays on the MiSeq and NextSeq NGS instruments for HIV, HCV, and human host cells for the DC CFAR investigator community.

Services and Instrumentation

  • Free Consultation Services

  • Targeted NGS of 4 regions within HIV-1 including PR, RT, IN and ENV. We also have the capability of analyzing the 46 drug resistant mutations (DRM) recognized by WHO.

  • Whole Genome Sequencing (WGS) and targeted sequencing of HCV.

  • RNA-Seq of host cells

To request laboratory services, please complete the DC CFAR Core Service Request Form or contact the Basic Sciences Core Manager. Additional information about available services can be found in the available laboratory documents. 

Leadership

The NGS Laboratory is directed by Dr. Jeanne A. Jordan, who has more than 18 years directing clinical microbiology, virology and molecular diagnostic lab services, and over 30 years of experience developing molecular diagnostic assays for detecting infectious diseases. She is a Professor in the Department of Epidemiology at the George Washington University. Dr. Kamwing Jair, Research Scientist/NGS Specialist, has over 10 years of NGS experience for Whole Genome and Targeted DNA Sequencing as well as RNA Sequencing at NIH NIAAA and GWU. Drs. Jordan and Jair are available to consult with DC CFAR investigators on the design, budget planning and development of relevant grant applications.