Recent research trends in immunology have opened new avenues of research and necessitated advanced flow cytometric instrumentation. Enumeration of central memory, long-lived, proliferating or polyfunctional cells are some of the few examples that require multiparametric flow cytometry (MFC) technology, since many markers are needed to discriminate each of these cell types from other populations of leukocytes. Highly multiplexed approaches such as MFC are critical exploratory tools to identify potential correlates of pathogenesis.
The Multiparametric Flow Cytometry Core Laboratory has a customized BD LSRFortessaTM X-20 with 4 lasers: Blue-488-50 mw, Red-640-100 mw, Violet-405-50 mw and the Yellow Green-561-50 mw; and includes a High-Throughput System (HTS) and a working station.
The instrument is available for booking. A basic training provided by the In-site Core Manager is required prior the first booking of instrument. Investigators are highly encouraged to apply for a Microgrant to cover the costs associated with the use of the core facility.
Dr. Bosque and Dr. Macedo are available to consult with DC CFAR investigators on the design and development of relevant grant applications. Assistance for the preparation, running and analysis of samples can be provided on an individual basis after consulting with the core director.
The Multiparametric Flow Cytometry Laboratory is directed by Dr. Alberto Bosque-Pardos.
- Basic Sciences
- Virus Detection and Analysis Molecular Virology
- Next Generation Sequencing
- RCMI Proteomics
- Artificial Intelligence and Drug Discovery
- Research Pathology
- Flow Cytometry and Cellular Immunology
- Multiparametric Flow Cytometry
- Biacore Molecular Interaction Shared Resource
- Microscopy and Imaging
- Center for Functional and Molecular Imaging
- Clinical and Population Sciences
- Social and Behavioral Sciences
- Request Core Services
- Acknowledge Funding and Services
District of Columbia Center for AIDS Research
Supported by the following Co-Funding and Participating Institutes: NIAID, NCI, NICHD, NIDCR, NHLBI, NIDA, NIMH, NIA, NIDDK, NINR, NIMHD, FIC, and OAR. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. (P30AI117970)