The Virus Detection and Analysis Molecular Virology Laboratory provides services related to analysis of various steps of HIV replication in target cells and the effects of anti-HIV compounds and treatments. We can evaluate stages of reverse transcription, integration, transcription, and viral release, analyze cell-to-cell transmission, and isolate virus from primary infected cells.
Services and Instrumentation
Virus detection and analysis services. Services include RT and p24 assays, in vitro infections, RT-PCR and PCR, virus infectivity assay, fusion assay, Western blot analysis for various HIV proteins and co-immunoprecipitation assays for HIV-related proteins.
Leadership
The laboratory is led by Dr. Michael Bukrinsky at the George Washington University where he is a Professor of Microbiology, Immunology and Tropical Diseases. Dr. Bukrinsky is available to consult with DC CFAR investigators on protocol development.
