The Virus Detection and Analysis Molecular Virology Laboratory provides services related to analysis of various steps of HIV replication in target cells and the effects of anti-HIV compounds and treatments. We can evaluate stages of reverse transcription, integration, transcription, and viral release, analyze cell-to-cell transmission, and isolate virus from primary infected cells.
Services and Instrumentation
Virus detection and analysis services. Services include RT and p24 assays, in vitro infections, RT-PCR and PCR, virus infectivity assay, fusion assay, Western blot analysis for various HIV proteins and co-immunoprecipitation assays for HIV-related proteins.
The laboratory is led by Dr. Michael Bukrinsky at the George Washington University where he is a Professor of Microbiology, Immunology and Tropical Diseases. Dr. Bukrinsky is available to consult with DC CFAR investigators on protocol development.
Supported by the following Co-Funding and Participating Institutes: NIAID, NCI, NICHD, NIDCR, NHLBI, NIDA, NIMH, NIA, NIDDK, NINR, NIMHD, FIC, and OAR. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. (P30AI117970)
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