Virus Detection and Analysis Molecular Virology
The Virus Detection and Analysis Molecular Virology Laboratory provides services related to analysis of various steps of HIV replication in target cells and the effects of anti-HIV compounds and treatments. We can evaluate stages of reverse transcription, integration, transcription, and viral release, analyze cell-to-cell transmission, and isolate virus from primary infected cells.
Services and Instrumentation
Virus detection and analysis services. Services include RT and p24 assays, in vitro infections, RT-PCR and PCR, virus infectivity assay, fusion assay, Western blot analysis for various HIV proteins and co-immunoprecipitation assays for HIV-related proteins.
The laboratory is led by Dr. Michael Bukrinsky at the George Washington University where he is a Professor of Microbiology, Immunology and Tropical Diseases. Dr. Bukrinsky is available to consult with DC CFAR investigators on protocol development.