DC CFAR investigator Jeanne Jordan, PhD, along with her colleagues at George Washington University and with the DC Cohort, has recently published an article in PLOS ONE entitled, "Validation of publicly-available software used in analyzing NGS data for HIV-1 drug resistance mutations and transmission networks in a Washington, DC, Cohort".
The article describes how the authors performed targeted, paired-end next-generation sequencing (NGS) of protease, reverse transcriptase and integrase amplicons to better understand HIV-1 drug resistance and potential transmission clusters among the DC Cohort participants. To do this, the authors used free, publicly available software that did not require extensive bioinformatics expertise. They found that common drug resistance mutations existed among individuals within a cluster, and in doing so validated a 'comprehensive, easy-to-use and affordable NGS approach for tracking HIV-1 drug resistance and identifying potential transmission clusters within the community."
Click here to read the full article.