RCMI Proteomics Core Laboratory
The RCMI Proteomics Core Laboratory, located at Howard University Hospital, provides services associated with analysis of an entire complement of proteins related to a particular process. The laboratory runs several research projects and provides collaborative consolidation of instrumentation, technical expertise, and support personnel to enhance the impact and availability of mass spectrometry and other analytical techniques.
Services and Instrumentation
- Protein identification analysis including peptide mapping by nano-LC-MS, peptide sequencing by nano-LC-MS/MS and database query using Proteome Discoverer 1.2 ($20/hour, HPLC-MS/MS running time is 2 hours for each sample)
- Protein phosphorylation analysis
- Label-free quantitative analysis of protein samples using SIEVE 2.1 (Thermo Scientific)
- Small molecules and their metabolites analysis
In addition to the available services, the RCMI Proteomics Core Laboratory has the following instrumentation:
- Prominence nano HPLC with autosampler (Shimadzu)
- LTQ-XL Orbitrap (nano-ESI mode) Mass Spectrometer (Thermo)
- Proteome Discoverer 1.2 with the licenses for SEQUEST and ZCore search engines
- SIEVE 2.1 software (Thermo Scientific) with license for Ingenuity
- Akta-Purifier HPLC (GE Healthcare)
- Luminescent spectrometers (Perkin Elmer and Promega)
- Plate reader (Bio-Rad)
- Flow cytometers: FACSVerse (Becton Dickinson) and Bioanalyzer 2100 (Agilent)
- XRS+ imaging station (Bio-Rad)
- SpeedVac (Savant)
- Bio-Plex (Bio-Rad)
- Hunter 2D Thin Layer Electrophoresis System (CBS)
To request core laboratory services, please complete the Core Service Request Form or contact the Core Manager. Click here to view a list of selected publications based on work done through the RCMI Proteomics service.
The laboratory is directed by Sergei Nekhai, PhD, Professor of Medicine and co-Directed by Tatiana Ammosova, PhD, Assistant Professsor at the Howard University College of Medicine. Dr. Nekhai and laboratory staff are available to assist DC CFAR investigators or train them in the use of laboratory instruments.