Multiparametric Flow Cytometry Core Laboratory

Recent research trends in immunology have opened new avenues of research and necessitated advanced flow cytometric instrumentation.

Enumeration of central memory, long-lived, proliferating or polyfunctional cells requires multiparametric flow cytometry (MFC) technology, since many markers are needed to discriminate each of these cell types from other populations of leukocytes.

Highly multiplexed approaches such as MFC are critical exploratory tools to identify potential correlates of pathogenesis.

Services and Instrumentation

The Multiparametric Flow Cytometry Core Laboratory has a BD customized Fortessa x-20 with 4 lasers, Blue-488-50mw, Red-640-100mw, Violet-405-50mw and the Yellow Green-561-50 mw, and includes a HTS system and a working station. The instrument is available for booking for those trained in its usage. The costs for use are currently being calculated and will be available shortly.

To request core laboratory services, please complete the Core Service Request Form or contact the Core Manager.

Leadership

The Multiparametric Flow Cytometry Core Laboratory is directed by Douglas Nixon, MD, PhD, who has more than 25 years of experience in immunovirology research. He is the Chair of Microbiology, Immunology and Tropical Diseases at the George Washington University.

Dr. Nixon and laboratory staff are available to consult with DC CFAR investigators on the design and development of relevant grant applications.

Core Manager

Isabella Kaser
kaser@gwu.edu

Laboratory Director